Honors and AP Biology students created yeast spheres to observe the catalase enzyme in action on Oct. 18.
They experimented with different concentrations and temperatures of a substrate solution to uncover the factors that influence enzymatic activity. Students first combined a yeast solution with sodium agitate to encapsulate the yeast in a gel-like material. Each group used syringes to create around 60 uniform yeast spheres by injecting drops of the solution into calcium lactate.
“We got to have hands-on experience to form the yeast structures,” AP Biology student Syna Sharma (11) said. “It was cool to see how the yeast reacted with the different concentrations of substrate and to test that ourselves.”
By timing how long it took for a yeast sphere to rise to the top of a hydrogen peroxide solution, students measured the effect of hydrogen peroxide concentration on the rate of the reaction. They dropped the yeast spheres individually into solutions of 0.3%, 0.6%, 1.5% and 3% hydrogen peroxide, which floated because of the production of oxygen gas.
“I love that this lab let students see reactions in the body happen, which we don’t often do with our biochemical experiments,” AP Biology teacher Matthew Harley said. “It was more visual because they could see hydrogen peroxide break down, and also it was fun to see them making spheres and bringing out their inner child with this lab.”
Students also tested the effect of temperature on the rate of the reaction by using heated, chilled and room temperature hydrogen peroxide solutions and timing the reaction rate.
“Even though this was a roundabout way to learn about enzymes since we already learned the principles in chemistry, it was great to see the reaction and the bubbles,” AP Biology student Timothy Deng (11) said. “If all classes were lectures all the time, that would be quite dull. It helped me learn in a way that I wouldn’t have if I was just listening since I was able to see the biological element in real life.”